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myc p31 comet addgene  (Addgene inc)


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    Addgene inc myc p31 comet addgene
    Fig. 5 Cells expressing a linear ubiquitination-deficient p31comet exhibit prolonged mitosis. A. Immunoblot (IB) analysis of whole cell lysates (WCL) and anti-ubiquitin immunoprecipitates (IP) derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. B. IB analysis of WCL and anti-HA IP derived from HEK293 cells. C. IB analysis of WCL and anti-HA IP derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. D. IB analysis of WCL and anti-HA IP derived from 293 T cells transfected with Myc-p31comet, HA-PLK1, His-UB and Flag-HOIP as indicated. E–F. Time-lapse microscopy of sgp31-H2B-YFP-HeLa cells stably expressing WT or 5KR-p31comet. E Representative images showing that <t>5KR-p31-HeLa</t> cells took a significantly longer time to complete the mitosis. F Quantification of the recorded mitotic duration times (minutes) of WT- and 5KR-p31comet expressing cells, n = 50, Student’s t-test. (See also Figure S5)
    Myc P31 Comet Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+p31+comet+addgene/pcDNA5+FRT+TO+myc+p31Comet+(Plasmid+%2359833)/pm40462232-114-54-57
    Average 85 stars, based on 2 article reviews
    myc p31 comet addgene - by Bioz Stars, 2026-10
    85/100 stars

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    1) Product Images from "Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation."

    Article Title: Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation.

    Journal: Cell & bioscience

    doi: 10.1186/s13578-025-01416-8

    Fig. 5 Cells expressing a linear ubiquitination-deficient p31comet exhibit prolonged mitosis. A. Immunoblot (IB) analysis of whole cell lysates (WCL) and anti-ubiquitin immunoprecipitates (IP) derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. B. IB analysis of WCL and anti-HA IP derived from HEK293 cells. C. IB analysis of WCL and anti-HA IP derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. D. IB analysis of WCL and anti-HA IP derived from 293 T cells transfected with Myc-p31comet, HA-PLK1, His-UB and Flag-HOIP as indicated. E–F. Time-lapse microscopy of sgp31-H2B-YFP-HeLa cells stably expressing WT or 5KR-p31comet. E Representative images showing that 5KR-p31-HeLa cells took a significantly longer time to complete the mitosis. F Quantification of the recorded mitotic duration times (minutes) of WT- and 5KR-p31comet expressing cells, n = 50, Student’s t-test. (See also Figure S5)
    Figure Legend Snippet: Fig. 5 Cells expressing a linear ubiquitination-deficient p31comet exhibit prolonged mitosis. A. Immunoblot (IB) analysis of whole cell lysates (WCL) and anti-ubiquitin immunoprecipitates (IP) derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. B. IB analysis of WCL and anti-HA IP derived from HEK293 cells. C. IB analysis of WCL and anti-HA IP derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. D. IB analysis of WCL and anti-HA IP derived from 293 T cells transfected with Myc-p31comet, HA-PLK1, His-UB and Flag-HOIP as indicated. E–F. Time-lapse microscopy of sgp31-H2B-YFP-HeLa cells stably expressing WT or 5KR-p31comet. E Representative images showing that 5KR-p31-HeLa cells took a significantly longer time to complete the mitosis. F Quantification of the recorded mitotic duration times (minutes) of WT- and 5KR-p31comet expressing cells, n = 50, Student’s t-test. (See also Figure S5)

    Techniques Used: Expressing, Ubiquitin Proteomics, Western Blot, Derivative Assay, Transfection, Time-lapse Microscopy, Stable Transfection

    Related Articles

    Expressing:

    Article Title: Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation.
    Article Snippet: HOIL-1L MilliporeSigma clone 2E2 MABC576 TUBULIN MilliporeSigma T-5168 VINCULIN MilliporeSigma V-4505 Flag (polyclonal) MilliporeSigma F-2425 Flag (monoclonal) MilliporeSigma F-3165 anti-Flag agarose beads MilliporeSigma A-2220 anti-HA agarose beads MilliporeSigma A-2095 peroxidase-conjugated anti-mouse secondary antibody MilliporeSigma A-4416 peroxidase-conjugated anti-rabbit secondary antibody MilliporeSigma A-4914 Plasmids pCMV3flag8HOIP Addgene 50,015 pCMV3flag8HOIL-1L Addgene 50,016 pCMV3-flag8-SHARPIN Addgene 50,014 pcDNA5 FRT TO myc p31 Comet Addgene 59,833 pERB160-hMAD1 Addgene 58,285 pcDNA5-EGFP-AIDBubR1 Addgene 47,330 VL3 (Flag-Mad2) Addgene 16,047 pcDNA3 HA human NEMO Addgene 13,512 pLKO.1-puro Addgene 8453 lentiCRISPRv2-puro Addgene 98,290 pHK-mTRIP13 Dr. Kevin D. Corbett, UCSD pcDNA3-6His-ubiquitin and mutants PMID: 31,015,455

    Ubiquitin Proteomics:

    Article Title: Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation.
    Article Snippet: HOIL-1L MilliporeSigma clone 2E2 MABC576 TUBULIN MilliporeSigma T-5168 VINCULIN MilliporeSigma V-4505 Flag (polyclonal) MilliporeSigma F-2425 Flag (monoclonal) MilliporeSigma F-3165 anti-Flag agarose beads MilliporeSigma A-2220 anti-HA agarose beads MilliporeSigma A-2095 peroxidase-conjugated anti-mouse secondary antibody MilliporeSigma A-4416 peroxidase-conjugated anti-rabbit secondary antibody MilliporeSigma A-4914 Plasmids pCMV3flag8HOIP Addgene 50,015 pCMV3flag8HOIL-1L Addgene 50,016 pCMV3-flag8-SHARPIN Addgene 50,014 pcDNA5 FRT TO myc p31 Comet Addgene 59,833 pERB160-hMAD1 Addgene 58,285 pcDNA5-EGFP-AIDBubR1 Addgene 47,330 VL3 (Flag-Mad2) Addgene 16,047 pcDNA3 HA human NEMO Addgene 13,512 pLKO.1-puro Addgene 8453 lentiCRISPRv2-puro Addgene 98,290 pHK-mTRIP13 Dr. Kevin D. Corbett, UCSD pcDNA3-6His-ubiquitin and mutants PMID: 31,015,455

    Western Blot:

    Article Title: Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation.
    Article Snippet: HOIL-1L MilliporeSigma clone 2E2 MABC576 TUBULIN MilliporeSigma T-5168 VINCULIN MilliporeSigma V-4505 Flag (polyclonal) MilliporeSigma F-2425 Flag (monoclonal) MilliporeSigma F-3165 anti-Flag agarose beads MilliporeSigma A-2220 anti-HA agarose beads MilliporeSigma A-2095 peroxidase-conjugated anti-mouse secondary antibody MilliporeSigma A-4416 peroxidase-conjugated anti-rabbit secondary antibody MilliporeSigma A-4914 Plasmids pCMV3flag8HOIP Addgene 50,015 pCMV3flag8HOIL-1L Addgene 50,016 pCMV3-flag8-SHARPIN Addgene 50,014 pcDNA5 FRT TO myc p31 Comet Addgene 59,833 pERB160-hMAD1 Addgene 58,285 pcDNA5-EGFP-AIDBubR1 Addgene 47,330 VL3 (Flag-Mad2) Addgene 16,047 pcDNA3 HA human NEMO Addgene 13,512 pLKO.1-puro Addgene 8453 lentiCRISPRv2-puro Addgene 98,290 pHK-mTRIP13 Dr. Kevin D. Corbett, UCSD pcDNA3-6His-ubiquitin and mutants PMID: 31,015,455

    Derivative Assay:

    Article Title: Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation.
    Article Snippet: HOIL-1L MilliporeSigma clone 2E2 MABC576 TUBULIN MilliporeSigma T-5168 VINCULIN MilliporeSigma V-4505 Flag (polyclonal) MilliporeSigma F-2425 Flag (monoclonal) MilliporeSigma F-3165 anti-Flag agarose beads MilliporeSigma A-2220 anti-HA agarose beads MilliporeSigma A-2095 peroxidase-conjugated anti-mouse secondary antibody MilliporeSigma A-4416 peroxidase-conjugated anti-rabbit secondary antibody MilliporeSigma A-4914 Plasmids pCMV3flag8HOIP Addgene 50,015 pCMV3flag8HOIL-1L Addgene 50,016 pCMV3-flag8-SHARPIN Addgene 50,014 pcDNA5 FRT TO myc p31 Comet Addgene 59,833 pERB160-hMAD1 Addgene 58,285 pcDNA5-EGFP-AIDBubR1 Addgene 47,330 VL3 (Flag-Mad2) Addgene 16,047 pcDNA3 HA human NEMO Addgene 13,512 pLKO.1-puro Addgene 8453 lentiCRISPRv2-puro Addgene 98,290 pHK-mTRIP13 Dr. Kevin D. Corbett, UCSD pcDNA3-6His-ubiquitin and mutants PMID: 31,015,455

    Transfection:

    Article Title: Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation.
    Article Snippet: HOIL-1L MilliporeSigma clone 2E2 MABC576 TUBULIN MilliporeSigma T-5168 VINCULIN MilliporeSigma V-4505 Flag (polyclonal) MilliporeSigma F-2425 Flag (monoclonal) MilliporeSigma F-3165 anti-Flag agarose beads MilliporeSigma A-2220 anti-HA agarose beads MilliporeSigma A-2095 peroxidase-conjugated anti-mouse secondary antibody MilliporeSigma A-4416 peroxidase-conjugated anti-rabbit secondary antibody MilliporeSigma A-4914 Plasmids pCMV3flag8HOIP Addgene 50,015 pCMV3flag8HOIL-1L Addgene 50,016 pCMV3-flag8-SHARPIN Addgene 50,014 pcDNA5 FRT TO myc p31 Comet Addgene 59,833 pERB160-hMAD1 Addgene 58,285 pcDNA5-EGFP-AIDBubR1 Addgene 47,330 VL3 (Flag-Mad2) Addgene 16,047 pcDNA3 HA human NEMO Addgene 13,512 pLKO.1-puro Addgene 8453 lentiCRISPRv2-puro Addgene 98,290 pHK-mTRIP13 Dr. Kevin D. Corbett, UCSD pcDNA3-6His-ubiquitin and mutants PMID: 31,015,455

    Time-lapse Microscopy:

    Article Title: Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation.
    Article Snippet: HOIL-1L MilliporeSigma clone 2E2 MABC576 TUBULIN MilliporeSigma T-5168 VINCULIN MilliporeSigma V-4505 Flag (polyclonal) MilliporeSigma F-2425 Flag (monoclonal) MilliporeSigma F-3165 anti-Flag agarose beads MilliporeSigma A-2220 anti-HA agarose beads MilliporeSigma A-2095 peroxidase-conjugated anti-mouse secondary antibody MilliporeSigma A-4416 peroxidase-conjugated anti-rabbit secondary antibody MilliporeSigma A-4914 Plasmids pCMV3flag8HOIP Addgene 50,015 pCMV3flag8HOIL-1L Addgene 50,016 pCMV3-flag8-SHARPIN Addgene 50,014 pcDNA5 FRT TO myc p31 Comet Addgene 59,833 pERB160-hMAD1 Addgene 58,285 pcDNA5-EGFP-AIDBubR1 Addgene 47,330 VL3 (Flag-Mad2) Addgene 16,047 pcDNA3 HA human NEMO Addgene 13,512 pLKO.1-puro Addgene 8453 lentiCRISPRv2-puro Addgene 98,290 pHK-mTRIP13 Dr. Kevin D. Corbett, UCSD pcDNA3-6His-ubiquitin and mutants PMID: 31,015,455

    Stable Transfection:

    Article Title: Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation.
    Article Snippet: HOIL-1L MilliporeSigma clone 2E2 MABC576 TUBULIN MilliporeSigma T-5168 VINCULIN MilliporeSigma V-4505 Flag (polyclonal) MilliporeSigma F-2425 Flag (monoclonal) MilliporeSigma F-3165 anti-Flag agarose beads MilliporeSigma A-2220 anti-HA agarose beads MilliporeSigma A-2095 peroxidase-conjugated anti-mouse secondary antibody MilliporeSigma A-4416 peroxidase-conjugated anti-rabbit secondary antibody MilliporeSigma A-4914 Plasmids pCMV3flag8HOIP Addgene 50,015 pCMV3flag8HOIL-1L Addgene 50,016 pCMV3-flag8-SHARPIN Addgene 50,014 pcDNA5 FRT TO myc p31 Comet Addgene 59,833 pERB160-hMAD1 Addgene 58,285 pcDNA5-EGFP-AIDBubR1 Addgene 47,330 VL3 (Flag-Mad2) Addgene 16,047 pcDNA3 HA human NEMO Addgene 13,512 pLKO.1-puro Addgene 8453 lentiCRISPRv2-puro Addgene 98,290 pHK-mTRIP13 Dr. Kevin D. Corbett, UCSD pcDNA3-6His-ubiquitin and mutants PMID: 31,015,455



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    myc p31 comet addgene  (Addgene inc)
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    Addgene inc myc p31 comet addgene
    Fig. 5 Cells expressing a linear ubiquitination-deficient p31comet exhibit prolonged mitosis. A. Immunoblot (IB) analysis of whole cell lysates (WCL) and anti-ubiquitin immunoprecipitates (IP) derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. B. IB analysis of WCL and anti-HA IP derived from HEK293 cells. C. IB analysis of WCL and anti-HA IP derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. D. IB analysis of WCL and anti-HA IP derived from 293 T cells transfected with Myc-p31comet, HA-PLK1, His-UB and Flag-HOIP as indicated. E–F. Time-lapse microscopy of sgp31-H2B-YFP-HeLa cells stably expressing WT or 5KR-p31comet. E Representative images showing that <t>5KR-p31-HeLa</t> cells took a significantly longer time to complete the mitosis. F Quantification of the recorded mitotic duration times (minutes) of WT- and 5KR-p31comet expressing cells, n = 50, Student’s t-test. (See also Figure S5)
    Myc P31 Comet Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+p31+comet+addgene/pcDNA5+FRT+TO+myc+p31Comet+(Plasmid+%2359833)/pm40462232-114-54-57
    Average 85 stars, based on 1 article reviews
    myc p31 comet addgene - by Bioz Stars, 2026-10
    85/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 5 Cells expressing a linear ubiquitination-deficient p31comet exhibit prolonged mitosis. A. Immunoblot (IB) analysis of whole cell lysates (WCL) and anti-ubiquitin immunoprecipitates (IP) derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. B. IB analysis of WCL and anti-HA IP derived from HEK293 cells. C. IB analysis of WCL and anti-HA IP derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. D. IB analysis of WCL and anti-HA IP derived from 293 T cells transfected with Myc-p31comet, HA-PLK1, His-UB and Flag-HOIP as indicated. E–F. Time-lapse microscopy of sgp31-H2B-YFP-HeLa cells stably expressing WT or 5KR-p31comet. E Representative images showing that 5KR-p31-HeLa cells took a significantly longer time to complete the mitosis. F Quantification of the recorded mitotic duration times (minutes) of WT- and 5KR-p31comet expressing cells, n = 50, Student’s t-test. (See also Figure S5)

    Journal: Cell & bioscience

    Article Title: Linear ubiquitination of p31 comet by HOIP couples cytokine response with mitotic regulation.

    doi: 10.1186/s13578-025-01416-8

    Figure Lengend Snippet: Fig. 5 Cells expressing a linear ubiquitination-deficient p31comet exhibit prolonged mitosis. A. Immunoblot (IB) analysis of whole cell lysates (WCL) and anti-ubiquitin immunoprecipitates (IP) derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. B. IB analysis of WCL and anti-HA IP derived from HEK293 cells. C. IB analysis of WCL and anti-HA IP derived from HEK293 cells. Cells were treated with 50 ng∙ml−1 TNFα for 10 min as indicated before harvest. D. IB analysis of WCL and anti-HA IP derived from 293 T cells transfected with Myc-p31comet, HA-PLK1, His-UB and Flag-HOIP as indicated. E–F. Time-lapse microscopy of sgp31-H2B-YFP-HeLa cells stably expressing WT or 5KR-p31comet. E Representative images showing that 5KR-p31-HeLa cells took a significantly longer time to complete the mitosis. F Quantification of the recorded mitotic duration times (minutes) of WT- and 5KR-p31comet expressing cells, n = 50, Student’s t-test. (See also Figure S5)

    Article Snippet: HOIL-1L MilliporeSigma clone 2E2 MABC576 TUBULIN MilliporeSigma T-5168 VINCULIN MilliporeSigma V-4505 Flag (polyclonal) MilliporeSigma F-2425 Flag (monoclonal) MilliporeSigma F-3165 anti-Flag agarose beads MilliporeSigma A-2220 anti-HA agarose beads MilliporeSigma A-2095 peroxidase-conjugated anti-mouse secondary antibody MilliporeSigma A-4416 peroxidase-conjugated anti-rabbit secondary antibody MilliporeSigma A-4914 Plasmids pCMV3flag8HOIP Addgene 50,015 pCMV3flag8HOIL-1L Addgene 50,016 pCMV3-flag8-SHARPIN Addgene 50,014 pcDNA5 FRT TO myc p31 Comet Addgene 59,833 pERB160-hMAD1 Addgene 58,285 pcDNA5-EGFP-AIDBubR1 Addgene 47,330 VL3 (Flag-Mad2) Addgene 16,047 pcDNA3 HA human NEMO Addgene 13,512 pLKO.1-puro Addgene 8453 lentiCRISPRv2-puro Addgene 98,290 pHK-mTRIP13 Dr. Kevin D. Corbett, UCSD pcDNA3-6His-ubiquitin and mutants PMID: 31,015,455

    Techniques: Expressing, Ubiquitin Proteomics, Western Blot, Derivative Assay, Transfection, Time-lapse Microscopy, Stable Transfection